目的 研究祁州漏芦水提物对四氯化碳诱发的急性肝损伤小鼠肝脏氧化应激和DNA损伤的影响。方法 将小鼠随机分为正常对照组、模型对照组、联苯双脂组及祁州漏芦水提物高、低剂量组。每日给药1次,连续7 d。实验末期,腹腔注射四氯化碳建立急性肝损伤模型。比色法检测血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶活性及肝脏过氧化脂质、丙二醛、还原型谷胱甘肽水平及过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶活性,电泳法检测小鼠肝细胞DNA损伤情况。结果 祁州漏芦水提物明显降低四氯化碳致急性肝损伤小鼠血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶活性;降低肝组织肝脏过氧化脂质和丙二醛水平;升高肝组织过氧化氢酶、谷胱甘肽过氧化酶、总超氧化物歧化酶、Mn-超氧化物歧化酶活性和还原型谷胱甘肽水平;升高肝线粒体Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性;并减少肝细胞DNA损伤程度。结论 祁州漏芦水提物对四氯化碳致急性肝损伤具有保护作用,其机制可能与其降低肝组织氧化应激和DNA损伤有关。
Abstract
To investigate the effect of Rhaponticum uniflorum water extract (RUWE) on the oxidative stress and DNA damage of liver with an acute liver injury induced by carbon tetrachloride (CCl4) in mice. METHODS Mice were randomly assigned to the normal control, model, bifendate (BFD), as well as high and low dose groups of RUWE. Animals were treated once daily for 7 d. At the end of the experiment, CCl4 was given intraperitoneally to the mice of groups, then the alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid hydroperoxide (LOOH), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), Na+-K+-ATPase and Ca2+-Mg2+-ATPase were detected with the colorimetric method, and DNA damage was analyzed using the electrophoretic method. RESULTS The administration with RUWE reduced the serum activities of ALT and AST, decreased the LOOH and MDA contents of liver, increased the CAT, GSH-Px, total SOD, Mn-SOD activities and GSH level of liver, increased the Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities of liver mitochondria, and reduced the DNA damage of hepatocyte in mice with acute liver injury. CONCLUSION RUWE has protective effect on acute liver injury induced by CCl4 in mice, probably via reduction of the oxidative stress and DNA damage of liver tissue.
关键词
漏芦 /
四氯化碳 /
肝损伤 /
氧化应激 /
DNA损伤
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Key words
Rhaponticum uniflorum /
carbon tetrachloride (CCl4) /
liver injury /
oxidative stress /
DNA damage
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中图分类号：
R285.5
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参考文献
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脚注
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基金
浙江省中医药管理局项目(2010ZA063,2011ZB063);浙江省药学会医院药学专项科研基金项目(2012ZYY01)
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